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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has long been noticed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was observed to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a significant role in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, happen to be described. Two supplemental splice variants ended up reported but thorough nucleotide sequence info will not be nonetheless accessible.

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Blocking peptides are peptides that bind precisely to your goal antibody and block antibody binding. These peptide typically has the epitope regarded by the antibody.

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This information outlines even more doable optimization of cellular and immunoassay parameters to make sure the very best effects are...

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to make certain the absolute best final results are...

Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from your blocked antibody get more info versus the antibody alone, one can see which staining is particular.

Focus on Information Cyclin E belongs towards the highly conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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